Hela-CAS9 cell line

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Hela-CAS9 Order

Catalog#

YC-A012-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

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Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

Hela-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the Hela cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Hela-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
HeLa-CAS9	Cas9	14.15423965	-0.64070892
HeLa-CAS9	β-actin	14.79494858	-0.64070892
Hela	Cas9	30.20011902	15.75909424
Hela	β-actin	14.44102478	15.75909424

Cutting Efficiency Validation

Hela-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the HeLa-CAS9 stable cell pool which is electroporated by ATF2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hela			STR Info (Cell bank) Cell Line:HELA
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	13.3		12	13.3
D7S820	8	12		8	12
D16S539	9	10		9	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	9	10		9	10
D12S391	20	24	25.0
FGA	21	21
D2S1338	17	17
D21S11	27	28
D18S51	16	16
D8S1179	12	13
D3S1358	15	18
D6S1043	18	19
PENTAE	7	17
D19S433	13	14
PENTAD	8	15
D1S1656	12	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

Hela-CAS9

Catalog#

YC-A012-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Hela-CAS9 cell line

Ubigene